Electron Microscope?

Originally Posted by Polo

Whats an electron microscope?

A microscope that uses a beam of energized electrons to view objects at very very fine scales. Basically they use electrons instead of light.

I use them to see Mina's dick.

Originally Posted by Cyberdyne

well if you really want to know, I'm researching bacteriophages on my own time and as a hobby besides my regular work.
and not just phages but I'm also really interested in culturing pathogens/virus and manipulating their genomes. Electron microscopes are the only way to view them. Compound microscopes only see a small dot on the slide. :/

isnt that illegal?

Originally Posted by Swiftdude

isnt that illegal?

No, if you had gone to school you would obviously know.

In fact, your house may be harboring more than tens of thousands of pathogen specimens waiting to infect some unlucky, unhygienic human being.

Hell, pathogens can grow anywhere, even under your very own fingernails.

So no it is NOT illegal to culture pathogens. You must be thinking about biological warfare agents.

@Cyberdyne he also might have ment manipulating the genomes of pathogens,which is obviously not true,this rule only applies to humans.

Also how do you plan on changing the genome,to see which one works the best toward combating others or what?

Originally Posted by argusomega1

@Cyberdyne he also might have ment manipulating the genomes of pathogens,which is obviously not true,this rule only applies to humans.

Also how do you plan on changing the genome,to see which one works the best toward combating others or what?

Read up on bacteriophages. I do not think that I will actually need to manipulate genomes directly, rather have the phages do it themselves.
The phages that I have cultured are called DX phages, and what I have noticed is that they are extremely prone to mutation when transcribing it's viral RNA. As you know, RNA viruses have high mutation rates...so that wasn't surprising. They produce errors during reverse transcription that are embedded into both of the strands of DNA before integration.

Taking advantage of genome transplantation, I am going to experiment with infecting bacteria with synthetic genomes with the DX-phages. High mutation rate = good for evolution! ... at least that's my theory.

Originally Posted by Cyberdyne

Read up on bacteriophages. I do not think that I will actually need to manipulate genomes directly, rather have the phages do it themselves.
The phages that I have cultured are called DX phages, and what I have noticed is that they are extremely prone to mutation when transcribing it's viral RNA. As you know, RNA viruses have high mutation rates...so that wasn't surprising. They produce errors during reverse transcription that are embedded into both of the strands of DNA before integration.

Taking advantage of genome transplantation, I am going to experiment with infecting bacteria with synthetic genomes with the DX-phages. High mutation rate = good for evolution! ... at least that's my theory.

What is your experimental group? E coli?

Originally Posted by Azathth

What is your experimental group? E coli?

*Escherichia coli O157:H7
*Mycoplasma mycoides bacterium

It's very much so legal to do, and to store any specimens I keep mine in a freezer that stays at 243.15 Kelvin. At that temperature all cells in any single or multi-celled organism slows to a point where they age about eight times slower. They still don't last as long but I'm always afraid of contamination.

1-2 weeks for equipment delivery.

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